This experimental studies was to investigate the location of PNS and CNS labeled neurons following injection of 2% WGA-HRP and pseudorabies virus (PRV), Bartha strain, into the ductus deferens of rats. After survival times 4-5 days following injection of 2% WGA-HRP and PRV, the rats were perfused, and their brain, spinal cord, sympathetic ganglia and spinal ganglia were frozen sectioned (30㎛). These sections were stained by HRP histochemical and PRV immunohistochemical staining methods, and observed with light microscope. The results were as follows:
1. The location of sympathetic ganglia projecting to the deferens were observed in pelvic ganglion, inferior mesenteric ganglion and L 1-6 lumbar sympathetic ganglia. 2. The location of spinal ganglia projecting to the ductus deferens were observed in T13-L6 spinal ganglia. 3. The PRV labeled neurons projecting to the ductus deferens were observed in lateral spinal nucleus, lamina I, II and X of cervical segments. In thoracic segments, PRV labeled neurons were observed in dorsomedial part of lamina I, II and III, and dorsolateral part of lamina IV and V. Densely labeled neurons were observed in intermediolateral nucleus. In first lumbar segment, labeled neurons were observed in intermediolateral nucleus and dorsal commisural nucleus. In sixth lumbar segment and sacral segments, dense labeled neurons were observed in sacral parasympathetic nuc., lamina IX and X. 4. In the medulla oblongata, PRV labeled neurons projecting to the ductus deferens were observedin the trigeminal spinal nuc., A1 noradrenalin cells/C1 adrenalin cells/caudoventralateral reticular nuc., rostroventrolateral reticular nuc., area postrema, nuc. tractus solitarius, raphe obscurus nuc., raphe pallidus nuc., raphe magnus nuc., parapyamidal nuc., lateral reticular nuc., gigantocellular reticular nuc., 5. In the pons, ORV labeled neurons projecting to the ductus deferens were observed in parabrachial nuc., Kolliker-Puse nuc., locus coeruleus, subcoeruleus nuc., and A5 nordrenalin cells. 6. In midbrain, PRV labeled neurons projecting to the ductus deferens were observed in periaqueductal gray substance, substantia nigra and dorsal raphe nuc., 7. In the diencephalon, PRV labeled neurons projecting to the ductus deferens were observed in panaventricular hypothalamic nuc., lateral hypothalamic nuc., retrochiasmatic nuc., and ventromedial hypothalamic nuc., 8. In cerebrum, PRV labeled neurons projecting to the ductus deferens were observed in area 1 of parietal cortex. These results suggest that WGA-HRP labeled neurons of the spinal cord projecting to the rat ductus deferens might be the first-order neurons related to the viscero-somatic sensory and sympathetic postganglionic neurons, and PRV labeled neurons of the brain and spinal cord may be the second and third-order neurons response to the movement of smooth muscles in ductus deferens. These PRV labeled neurons may be central autonomic center related to the integration and modulation of reflex control linked to the sensory and motor system monitoring the internal environment. These observations provide evidence for previously unknown projections from ductus deference to spinal cord and brain which may be play an important neuroanatomical basic evidence in the regulation of ductus deferens function.
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